NAD+ / Glutathione / Vitamin C blend (nasal spray) (Nasal spray) dosing

What it is

This is a nasal spray holding three things at once. The amount of each per spray has not been published, so this page does not give one.

NAD+ stands for nicotinamide adenine dinucleotide. It is a coenzyme — a small helper molecule that enzymes need to do their jobs — present in every living cell, and central to turning food into usable energy and to repairing DNA. The amount your body makes starts falling around age 30.

Glutathione is your body's main built-in antioxidant — a substance that neutralises free radicals, unstable molecules that damage cells. It is also central to how the body clears out toxins.

Vitamin C (ascorbic acid) is a second antioxidant, and one of its jobs is regenerating other antioxidants after they have been used up — including glutathione. That is the logic of putting the three together: NAD+ for energy production, glutathione to soak up the damage, and vitamin C to keep the glutathione working.

It comes as a liquid in an atomizer — a pump bottle that breaks the liquid into a fine mist you breathe into your nose. Because it goes in through the lining of the nose, it skips the digestive system, which is why you do not need to be fasted. Store the atomizer in the fridge.

The documented information is limited to contraindications and dosing. No benefits list or side effects list has been published for the blend.

Fixed intranasal combination of NAD+, glutathione, and ascorbate. No per-spray concentrations have been published for any of the three, so none are given here.

NAD+ is the central redox coenzyme of intermediary metabolism and the obligate substrate of the sirtuins and PARPs; endogenous levels decline from roughly the third decade.

Glutathione is the principal endogenous thiol antioxidant and the substrate of phase II conjugation in xenobiotic clearance.

Ascorbate is a water-soluble antioxidant that, among other roles, recycles oxidised antioxidant species — the redox coupling with glutathione is the mechanistic rationale for including both.

The combination is therefore a bioenergetic arm plus a redox-buffering arm: NAD+ increases flux through pathways that generate reactive oxygen species, and the two antioxidants sit downstream of that.

Refrigerated atomizer, intranasal route, no fasted-state requirement. Onset is given as 15 minutes to 1 hour — notably tighter than the standalone intranasal NAD+ entry, which gives a few minutes to several hours. 12-week cycle with a 4-week washout.

What it does

What the blend does has not been documented; the available information covers contraindications, timing, technique, and dosing.

From the injectable NAD+ entry on this site, for the NAD+ component: it promotes healthy ageing, boosts energy, increases metabolism, protects brain cells, keeps mitochondria working (the tiny structures inside cells that turn food into energy), supports heart and blood vessel health, maintains thinking, and supports the immune system. There is no standalone glutathione or vitamin C entry on this site, so nothing more can be said about those two here.

There is also a warning tied to timing: morning dosing is preferred, because NAD+ may lead to increased energy and disturbed sleep. Anyone planning a second dose in the day should take that into account.

The technique is the same as for the other nasal sprays. Always split the dose between both nostrils as much as you can — that exposes more of the nasal lining, reduces how much runs down your throat and gets swallowed, and makes absorption more consistent, because airflow, congestion, mucus, and the normal cycling of your nostrils can make one side less effective than the other. Do not tilt your head back. Tip your head forward, angle the atomizer towards the outside of your nose, and sniff gently.

No pharmacodynamics have been documented for the blend. From the injectable NAD+ entry, for that component: healthy-ageing and lifespan effects, increased cellular energy output, increased metabolic rate, neuroprotection against oxidative and ischaemic stress, restored mitochondrial function, reduced atherosclerotic plaque, restored arterial elasticity, cognitive maintenance, improved muscle function, immune support, and sirtuin substrate provision. No standalone glutathione or vitamin C entry exists on this site, so neither antioxidant arm has documented content here.

The blend-specific guidance is chronobiological and technical. Morning preferred, with the reason stated explicitly: NAD+ may produce increased energy and sleep disturbance, and anyone adding a second daily dose should account for that. This is the clearest statement of the NAD+ sleep-disruption issue anywhere in the NAD+ entries on this site.

Administration technique: bilateral splitting to maximise mucosal surface area exposed, reduce pharyngeal runoff and swallowing loss, and buffer against the nasal cycle — the physiological alternation in congestion between nostrils. Head tipped forward, atomizer angled laterally toward the outer canthus, gentle sniff; never head-back, which drives solution into the nasopharynx to be swallowed.

Benefits

Evidence grades: what the labels mean

Human trials Supported by randomised or placebo-controlled human trials.
Limited human data Some human evidence, such as pilot studies, case reports or observational data, but no controlled trials.
Animal or lab only Shown in animal or cell studies only; not yet tested in people.
Anecdotal No published studies; based on user reports or theory.

Each grade reflects the strongest published support for that specific claim, not for the compound as a whole.

No benefits have been documented for the blend itself. The NAD+ items below are from the injectable NAD+ entry on this site.Anecdotal
From the injectable NAD+ entry: promotes healthy ageing and extends lifespan.Animal or lab only
From the injectable NAD+ entry: boosts energy.Anecdotal
From the injectable NAD+ entry: improves overall health.Anecdotal
From the injectable NAD+ entry: increases metabolism and supports weight loss.Animal or lab only
From the injectable NAD+ entry: protects brain cells from oxidative stress and from insufficient blood flow.Animal or lab only
From the injectable NAD+ entry: keeps mitochondria — the power plants inside cells — working properly.Animal or lab only
From the injectable NAD+ entry: reduces atherosclerosis, the plaque that builds up inside artery walls.Animal or lab only
From the injectable NAD+ entry: supports heart and blood vessel health by restoring the elasticity of arteries.Animal or lab only
From the injectable NAD+ entry: maintains or improves thinking.Animal or lab only
From the injectable NAD+ entry: improves muscle function.Animal or lab only
From the injectable NAD+ entry: supports immune system activity.Animal or lab only
From the injectable NAD+ entry: linked to sirtuins, proteins essential to healthy ageing, metabolic control, DNA repair, and keeping chromosomes stable.Animal or lab only
There is no standalone glutathione or vitamin C entry on this site, so no benefits are listed for those two components.Anecdotal
Specific to this route: no needles, no mixing, and no need to be fasted.Anecdotal

No benefits list has been documented for the blend itself. The NAD+ items are from the injectable NAD+ entry.Anecdotal
From the injectable NAD+ entry: healthy ageing and extended lifespan.Animal or lab only
From the injectable NAD+ entry: increased cellular energy output.Anecdotal
From the injectable NAD+ entry: improved overall health markers.Anecdotal
From the injectable NAD+ entry: increased metabolic rate and weight-loss support.Animal or lab only
From the injectable NAD+ entry: neuroprotection against oxidative stress and ischaemic insufficiency.Animal or lab only
From the injectable NAD+ entry: restored mitochondrial function.Animal or lab only
From the injectable NAD+ entry: reduced atherosclerotic plaque accumulation.Animal or lab only
From the injectable NAD+ entry: restored arterial elasticity.Animal or lab only
From the injectable NAD+ entry: cognitive maintenance and improvement.Animal or lab only
From the injectable NAD+ entry: improved muscle function and skeletal-muscle mitochondrial health.Animal or lab only
From the injectable NAD+ entry: immune support, itself NAD+-consuming on activation.Animal or lab only
From the injectable NAD+ entry: sirtuin substrate provision.Animal or lab only
No glutathione or vitamin C entry exists on this site; neither antioxidant arm has documented content here.Anecdotal
Route-specific: no reconstitution, no injection, no first-pass metabolism, no fasting requirement.Anecdotal

Reconstitution and dosing

Two to four sprays, once or twice per day, every day.

The cycle is 12 weeks followed by a 4-week break.

Morning is preferred, because NAD+ may give you increased energy and disturb your sleep. If you want a second dose in the day, keep that in mind when choosing the time.

Onset is 15 minutes to 1 hour. You do not need to be fasted, because the spray bypasses the digestive system. Keep the atomizer in the fridge.

Technique: always split the dose between both nostrils as much as possible. Do not tilt your head back. Tip your head forward, angle the atomizer towards the outside of your nose, and sniff gently.

The amount of NAD+, glutathione, or vitamin C in each spray has not been published, so no milligram figures can be given here.

2–4 sprays, once or twice daily, every day. 12-week cycle with a 4-week washout.

Morning preferred, with the reason stated: NAD+ may produce increased energy and sleep disturbance, and a second daily dose should be timed with that in mind. Onset 15 minutes to 1 hour. No fasted state required. Refrigerate the atomizer.

Bilateral splitting, head forward, atomizer angled toward the outer canthus, gentle sniff; never head-back.

No per-spray strengths are published for any of the three components, so exposure cannot be reconstructed and no comparison against the standalone intranasal NAD+ entry (5 mg per spray, 20–100 mg per day, 2–6 days per week) is possible. That entry's dosing is on a visibly different scale — up to 20 sprays against 4 to 8 here — which suggests a different NAD+ concentration per spray, though this has not been confirmed. Do not assume the two are interchangeable.

The cycle comparison is also worth noting: 12 weeks on here with a 4-week washout, against 12 weeks on with a 2-week washout for standalone intranasal NAD+.

Nasal atomizer — per-spray strengths not published

Cycle: 12 week cycle followed by a 4 week washout · Frequency: Daily. Morning preferred, because NAD+ may cause increased energy and sleep disturbance; take that into account before adding a second daily dose. Onset 15 minutes to 1 hour. Fasting not required; refrigerate the atomizer. Always split the dose between both nostrils, head tipped forward, never back

When	Dose	How often
Standard protocol	2–4 sprays	once or twice per day, every day; split between both nostrils

Who should avoid it

Anyone pregnant or nursing.
Anyone who has had a recent cancer diagnosis, or has an active one.
Anyone who is hypersensitive or allergic to glutathione or to NAD+.
The following are from the injectable NAD+ entry on this site, and are included here because they concern NAD+ itself rather than the route.
From the injectable NAD+ entry: anyone with diabetes should use caution — NAD+ may cause insulin resistance, high blood sugar, and difficulty handling glucose.
From the injectable NAD+ entry: anyone taking blood pressure medication — NAD+ may strengthen the effect and drop blood pressure too low.
From the injectable NAD+ entry: anyone on blood thinners such as Coumadin or warfarin — NAD+ may interfere with clotting.
From the injectable NAD+ entry: anyone taking antidepressants — NAD+ may worsen mood-related symptoms.
From the injectable NAD+ entry: anyone taking melatonin alongside it — the combination may cause heavy fatigue, grogginess, or brain fog.
From the injectable NAD+ entry, a caution rather than a contraindication: antioxidants such as quercetin and resveratrol can raise NAD+ levels five-fold, which can also magnify side effects — hot flushes, nausea, itching or burning skin, calf cramps, headaches, abdominal pain, and fatigue. Worth noting that this product contains two antioxidants of its own.
From the injectable NAD+ entry: taking more than 3,000 mg of NAD+ per month may lead to liver damage, kidney disease, and increased risk of blood vessel complications. The amount of NAD+ per spray has not been published, so that total cannot be worked out here.
No allergy warning has been documented for vitamin C, even though there are warnings about glutathione and NAD+ allergy.
Talk to a doctor before starting, and go through your full medication list with them.

Pregnancy or nursing.
Recent or active malignancy.
Hypersensitivity or allergy to glutathione or NAD+.
The remaining items are from the injectable NAD+ entry, included as compound-level rather than route-level considerations.
From the injectable NAD+ entry: diabetes — caution, given reported insulin resistance, hyperglycaemia, and glucose intolerance.
From the injectable NAD+ entry: antihypertensive therapy — potentiation and possible hypotension.
From the injectable NAD+ entry: anticoagulation with Coumadin/warfarin — interference with clotting.
From the injectable NAD+ entry: antidepressant therapy — possible worsening of mood-related symptoms.
From the injectable NAD+ entry: concurrent melatonin — amplified fatigue, grogginess, cognitive fog.
From the injectable NAD+ entry: quercetin and resveratrol raise NAD+ five-fold with proportionate amplification of side effects. Relevant here in that the product itself pairs NAD+ with two antioxidants, though neither of those two is the one the injectable entry names.
From the injectable NAD+ entry: cumulative NAD+ intake above 3,000 mg per month is associated with hepatic damage, renal disease, and increased vascular complication risk. Per-spray NAD+ content is not published for this product, so monthly exposure cannot be computed — a real gap if this is run alongside another NAD+ route.
Hypersensitivity to ascorbate has not been addressed, despite glutathione and NAD+ hypersensitivity both being listed.

Side effects

No side effects have been documented for the blend itself. The following are from the injectable NAD+ entry on this site, and cover the NAD+ component rather than this route or the antioxidants.
From the injectable NAD+ entry: diarrhoea.
From the injectable NAD+ entry: bruising easily.
From the injectable NAD+ entry: increased bleeding from wounds.
From the injectable NAD+ entry: nausea.
From the injectable NAD+ entry: vomiting.
From the injectable NAD+ entry: taking more than 3,000 mg of NAD+ a month may cause liver damage, kidney disease, and a higher risk of blood vessel problems.
The injection-site effects listed for injectable NAD+ do not apply here, since nothing is injected.
Given as a timing reason rather than as a side effect: NAD+ may lead to increased energy and disturbed sleep. That is why morning dosing is preferred, and why a second daily dose needs thinking about.
No nose-specific effects have been documented — irritation, dripping, or a taste in the throat if the spray runs back.

No side-effect list has been documented for the blend itself. The following are from the injectable NAD+ entry, covering the NAD+ component only.
From the injectable NAD+ entry: diarrhoea.
From the injectable NAD+ entry: easy bruising.
From the injectable NAD+ entry: increased wound bleeding.
From the injectable NAD+ entry: nausea.
From the injectable NAD+ entry: vomiting.
From the injectable NAD+ entry: NAD+ intake above 3,000 mg per month — hepatic damage, renal disease, increased vascular complication risk.
The injectable entry's route-specific items (intramuscular burning and histamine reactions, subcutaneous nodules) do not transfer.
Framed as dosing rationale rather than tolerability: NAD+ may produce increased energy and sleep disturbance, which is why morning dosing is preferred and why a second daily dose requires deliberation.
No local nasal tolerability data have been documented, and no glutathione- or ascorbate-attributable effects are given, since neither has an entry on this site.

User reports

User reports are individual experiences submitted by site visitors. They are not medical advice, are not verified for accuracy, and do not reflect Amino Reference's views. Read the evidence section above and talk to a clinician. Full disclaimer.

Stacking

NAD+ (nasal spray)
NAD+ on its own, in the same kind of nasal spray. That entry gives its strength — 5 mg per spray — where this one does not, and it goes up to a much higher daily amount. Do not run both at once without knowing what you are actually taking.
The NAD+ component alone by the same route, at a published 5 mg per spray and 20–100 mg daily on 2–6 days per week. Because this blend publishes no per-spray content, concurrent use of both cannot be reconciled against the 3,000 mg monthly ceiling.
NAD+ (injectable)
NAD+ as an injection. That entry supplies the benefits, side effects, and most of the contraindications on this page.
The NAD+ component by the subcutaneous or intramuscular route, 25–100 mg two to seven times weekly. It supplies all the NAD+ safety and efficacy content on this page.
Glutathione
One of the two antioxidants in this spray. There is no standalone glutathione entry on this site yet, so nothing about it appears in the lists above.
The thiol antioxidant component. No standalone entry exists on this site, so no benefit, side-effect, or contraindication content is available for it beyond the hypersensitivity warning on this page.
Vitamin C
The other antioxidant in this spray. It helps regenerate glutathione after it has been used up. There is no standalone vitamin C entry on this site.
Ascorbate, included for redox recycling of oxidised glutathione as much as for its own antioxidant activity. No standalone entry on this site, and notably no hypersensitivity warning for it on this page either.

Dosing figures have been reviewed and units are recomputed from the stated protocol.